Anatomical Neuropharmacology Unit

This is an historical archive of the activities of the MRC Anatomical Neuropharmacology Unit (MRC ANU) that operated at the University of Oxford from 1985 until March 2015. The MRC ANU established a reputation for world-leading research on the brain, for training new generations of scientists, and for engaging the general public in neuroscience. The successes of the MRC ANU are now built upon at the MRC Brain Network Dynamics Unit at the University of Oxford.

Differential internalisation of mGluR1 splice variants in response to agonist and phorbol esters in permanently transfected BHK cells.

FEBS Lett. 1997;418(1-2):83-6.

Differential internalisation of mGluR1 splice variants in response to agonist and phorbol esters in permanently transfected BHK cells.

Ciruela F, McIlhinney RAJ

Abstract:

The internalisation of metabotropic glutamate receptor (mGluR1alpha) and its splice variant (mGluR1beta), in response to agonist and phorbol esters (PMA), has been studied. Both mGluR1alpha and mGluR1beta exhibit a similar rate of internalisation following PMA treatment, with a shift in their distribution from plasma membrane to endosome-enriched membrane fractions. Agonist challenge however caused a rapid loss, within 5-10 min, of mGluR1beta but not mGluR1alpha from the cell surface. These results show that the two forms of mGluR1 show different internalisation responses to agonist and suggest that the C-terminal region of the molecule plays an important role in this phenomenon.

Research and Techniques:

Receptors, Metabotropic Glutamate

Recombinant Fusion Proteins

Tetradecanoylphorbol Acetate

Transfection

Differential internalisation of mGluR1 splice variants in response to agonist and phorbol esters in permanently transfected BHK cells.

You are here

Differential internalisation of mGluR1 splice variants in response to agonist and phorbol esters in permanently transfected BHK cells.